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normal human neonatal foreskin fibroblasts ccd 1064sk  (ATCC)


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    ATCC normal human neonatal foreskin fibroblasts ccd 1064sk
    Normal Human Neonatal Foreskin Fibroblasts Ccd 1064sk, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 119 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/neonatal+foreskin+fibroblasts/CCD-1064Sk/pm41906696-92-0-7
    Average 95 stars, based on 119 article reviews
    normal human neonatal foreskin fibroblasts ccd 1064sk - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Transfection:

    Article Title: Methods for reprogramming cells and uses thereof
    Article Snippet: .. Human keratinocytes (Invitrogen, Cat. #12332-011), CD34+ hematopoietic cells (StemCell Technologies, Cat. #MPB015F), and neonatal foreskin fibroblasts (HFF, American Type Culture Collection, Cat. #CRL-2097)) were transfected with pCMV-MSI1-2A-Ngn2 and pCMV6-XL5-MBD2 and grown in neural stem cell medium supplemented with EGF and FGF2 (NeuroCult Proliferation Kit Cat. #5751, StemCell Technologies) on CELLStartTM (Invitrogen, Cat. #A10142-01) coated plates at 37° C., 10% CO2, 5% O2 and supplemented with VPA and Noggin for the first 6 days. .. Human keratinocytes (Invitrogen, Cat. #12332-011), CD34+ hematopoietic cells (StemCell Technologies, Cat. #MPB015F), and neonatal foreskin fibroblasts (HFF, American Type Culture Collection, Cat. #CRL-2097)) were transfected with pCMV-MSI1-2A-Ngn2 and pCMV6-XL5-MBD2 and grown in neural stem cell medium supplemented with EGF and FGF2 (NeuroCult Proliferation Kit Cat. #5751, StemCell Technologies) on CELLStart™ (Invitrogen, Cat. #A10142-01) coated plates at 37° C., 10% CO2, 5% O2 and supplemented with VPA and Noggin for the first 6 days.

    Article Title: Methods for reprogramming cells and uses thereof
    Article Snippet: Human keratinocytes (Invitrogen, Cat. #12332-011), CD34+ hematopoietic cells (StemCell Technologies, Cat. #MPB015F), and neonatal foreskin fibroblasts (HFF, American Type Culture Collection, Cat. #CRL-2097)) were transfected with pCMV-MSI1-2A-Ngn2 and pCMV6-XL5-MBD2 and grown in neural stem cell medium supplemented with EGF and FGF2 (NeuroCult Proliferation Kit Cat. #5751, StemCell Technologies) on CELLStartTM (Invitrogen, Cat. #A10142-01) coated plates at 37° C., 10% CO2, 5% O2 and supplemented with VPA and Noggin for the first 6 days. .. Human keratinocytes (Invitrogen, Cat. #12332-011), CD34+ hematopoietic cells (StemCell Technologies, Cat. #MPB015F), and neonatal foreskin fibroblasts (HFF, American Type Culture Collection, Cat. #CRL-2097)) were transfected with pCMV-MSI1-2A-Ngn2 and pCMV6-XL5-MBD2 and grown in neural stem cell medium supplemented with EGF and FGF2 (NeuroCult Proliferation Kit Cat. #5751, StemCell Technologies) on CELLStart™ (Invitrogen, Cat. #A10142-01) coated plates at 37° C., 10% CO2, 5% O2 and supplemented with VPA and Noggin for the first 6 days. ..

    Cell Culture:

    Article Title: Epitranscriptomic regulation of cortical neurogenesis via Mettl8-dependent mitochondrial tRNA m 3 C modification.
    Article Snippet: PrimaryNPCswere cultured (105/well) in platespre-coatedwith 1%Matrigel and in theDMEM/F12mediumcontaining 2%B27supplement, 1%N2supplement, bFGF (20 ng/mL), EGF (20 ng/mL), 1%GlutaMAX, 1% P/S with digestion using Accutase (ThermoFisher) for passaging. .. For analysis of neural stem cell maintenance, freshly dissected primaryNPCswere seeded (2x105/well) on plates pre-coatedwith 20 ng/mLpoly-D-lysine (pdL) and cultured first inDMEM/10%FBS/ 1%P/S for 2 hours and then in theDMEM/F12mediumcontaining 1%N2supplement, bFGF (20 ng/mL), 1%GlutaMAX, 1%P/S for 48 hours.37 The founder human iPSC line (C1-2) used in the current study was previously generated from male neonatal foreskin fibroblasts from ATCC (CRL-2097) and fully characterized.58,67–69 Generation of iPSC lines followed institutional IRB and ISCRO guidelines and was approved by Johns Hopkins University School of Medicine. .. For analysis of neural stem cell maintenance, freshly dissected primaryNPCswere seeded (2x105/well) on plates pre-coatedwith 20 ng/mLpoly-D-lysine (pdL) and cultured first inDMEM/10%FBS/ 1%P/S for 2 hours and then in theDMEM/F12mediumcontaining 1%N2supplement, bFGF (20 ng/mL), 1%GlutaMAX, 1%P/S for 48 hours.37 The founder human iPSC line (C1-2) used in the current study was previously generated from male neonatal foreskin fibroblasts from ATCC (CRL-2097) and fully characterized.58,67–69 Generation of iPSC lines followed institutional IRB and ISCRO guidelines and was approved by Johns Hopkins University School of Medicine.

    Generated:

    Article Title: Epitranscriptomic regulation of cortical neurogenesis via Mettl8-dependent mitochondrial tRNA m 3 C modification.
    Article Snippet: PrimaryNPCswere cultured (105/well) in platespre-coatedwith 1%Matrigel and in theDMEM/F12mediumcontaining 2%B27supplement, 1%N2supplement, bFGF (20 ng/mL), EGF (20 ng/mL), 1%GlutaMAX, 1% P/S with digestion using Accutase (ThermoFisher) for passaging. .. For analysis of neural stem cell maintenance, freshly dissected primaryNPCswere seeded (2x105/well) on plates pre-coatedwith 20 ng/mLpoly-D-lysine (pdL) and cultured first inDMEM/10%FBS/ 1%P/S for 2 hours and then in theDMEM/F12mediumcontaining 1%N2supplement, bFGF (20 ng/mL), 1%GlutaMAX, 1%P/S for 48 hours.37 The founder human iPSC line (C1-2) used in the current study was previously generated from male neonatal foreskin fibroblasts from ATCC (CRL-2097) and fully characterized.58,67–69 Generation of iPSC lines followed institutional IRB and ISCRO guidelines and was approved by Johns Hopkins University School of Medicine. .. For analysis of neural stem cell maintenance, freshly dissected primaryNPCswere seeded (2x105/well) on plates pre-coatedwith 20 ng/mLpoly-D-lysine (pdL) and cultured first inDMEM/10%FBS/ 1%P/S for 2 hours and then in theDMEM/F12mediumcontaining 1%N2supplement, bFGF (20 ng/mL), 1%GlutaMAX, 1%P/S for 48 hours.37 The founder human iPSC line (C1-2) used in the current study was previously generated from male neonatal foreskin fibroblasts from ATCC (CRL-2097) and fully characterized.58,67–69 Generation of iPSC lines followed institutional IRB and ISCRO guidelines and was approved by Johns Hopkins University School of Medicine.

    Article Title: Human trunk embryoids with patterned anterior-posterior and dorsal-ventral body axes: utility for understanding human development and disease
    Article Snippet: .. The following human ESC and iPSC lines were used: wild type H9-hESC (Sex: female, WiCell, WAe009-A), K3-iPSC (Sex: male) generated from human neonatal foreskin fibroblasts (ATCC, PCS-201-010), H9-hESC line carrying IRES-mClover3 allele in the 3’ UTR of PAX3 and IRES-mScarlet allele in the 3’ UTR of EBF2 ( PAX3:mClover3; EBF2:mScarlet dual reporter ) , H9-hESC line carrying IRES-mScarlet allele in the 3’ UTR of NKX1-2 (NKX1-2:mScarlet reporter), H9-hESC line carrying IRES-mClover3 allele in the 3’ UTR of PAX3 and IRES-mScarlet allele in the 3’ UTR of NOTO ( NOTO:mClover3 reporter ), H9-hESC line carrying IRES-mClover3 allele in the 3’ UTR of MYF5 ( MYF5:mClover3 reporter), and H9-hESC derived NOTO-LOF, VANGL1-LOF, VANGL2-LOF, and VANGL1/2-LOF ( ). .. H9-hESC lines and K3-iPSCs were routinely cultured and passaged as described previously., Briefly, 5 × 10 4 /cm 2 cells were seeded onto culture-treated petri dishes coated with 1:200 diluted Geltrex (Thermo, A1413302).

    Derivative Assay:

    Article Title: Optimized prime editing of the Alzheimer’s disease-associated APOE4 mutation
    Article Snippet: iPSC line derivation 42 The R-iPS 1J cell line (internal identifier) were generated internally at AstraZeneca and not made 43 available for public use. .. The cells were derived from human neonatal foreskin fibroblasts (BJ CRL-44 2522, ATCC) with a normal diploid karyotype and reprogrammed using Yamanaka factors via 45 retroviral transduction. .. 46 iPSC culture 47 iPSC cells were maintained in Cellartis DEF-CS 500 Culture System (Y30010, Takara Bio) on DEF-48 CS COAT-1 (Y30012, Takara Bio), diluted 1:20 in PBS with Ca2+ & Mg2+, coated cell culture plates.

    Article Title: Optimized prime editing of the Alzheimer’s disease-associated APOE4 mutation
    Article Snippet: 39 40 Supplemental Experimental Procedures 41 iPSC line derivation 42 The R-iPS 1J cell line (internal identifier) were generated internally at AstraZeneca and not made 43 available for public use. .. The cells were derived from human neonatal foreskin fibroblasts (BJ CRL-44 2522, ATCC) with a normal diploid karyotype and reprogrammed using Yamanaka factors via 45 retroviral transduction. .. 46 iPSC culture 47 iPSC cells were maintained in Cellartis DEF-CS 500 Culture System (Y30010, Takara Bio) on DEF-48 CS COAT-1 (Y30012, Takara Bio), diluted 1:20 in PBS with Ca2+ & Mg2+, coated cell culture plates.

    Article Title: Human trunk embryoids with patterned anterior-posterior and dorsal-ventral body axes: utility for understanding human development and disease
    Article Snippet: .. The following human ESC and iPSC lines were used: wild type H9-hESC (Sex: female, WiCell, WAe009-A), K3-iPSC (Sex: male) generated from human neonatal foreskin fibroblasts (ATCC, PCS-201-010), H9-hESC line carrying IRES-mClover3 allele in the 3’ UTR of PAX3 and IRES-mScarlet allele in the 3’ UTR of EBF2 ( PAX3:mClover3; EBF2:mScarlet dual reporter ) , H9-hESC line carrying IRES-mScarlet allele in the 3’ UTR of NKX1-2 (NKX1-2:mScarlet reporter), H9-hESC line carrying IRES-mClover3 allele in the 3’ UTR of PAX3 and IRES-mScarlet allele in the 3’ UTR of NOTO ( NOTO:mClover3 reporter ), H9-hESC line carrying IRES-mClover3 allele in the 3’ UTR of MYF5 ( MYF5:mClover3 reporter), and H9-hESC derived NOTO-LOF, VANGL1-LOF, VANGL2-LOF, and VANGL1/2-LOF ( ). .. H9-hESC lines and K3-iPSCs were routinely cultured and passaged as described previously., Briefly, 5 × 10 4 /cm 2 cells were seeded onto culture-treated petri dishes coated with 1:200 diluted Geltrex (Thermo, A1413302).

    Article Title: Human cortico-vascular assembloids reveal a CELF2-AHNAK-dependent switch from neuronal to endothelial tropism in glioblastoma cells
    Article Snippet: .. HMGU1 hiPSCs, derived from neonatal foreskin fibroblasts (ATCC CRL-2522, BJ), were kindly provided by Dr. Drukker (HMGU, Germany) under a Material Transfer Agreement (MTA), and confirmed mycoplasma-free. .. Cells were maintained on Matrigel-coated plates (Corning, 354234) in mTeSR1 medium (STEMCELL Technologies, #85850) with daily medium changes.

    Retroviral:

    Article Title: Optimized prime editing of the Alzheimer’s disease-associated APOE4 mutation
    Article Snippet: iPSC line derivation 42 The R-iPS 1J cell line (internal identifier) were generated internally at AstraZeneca and not made 43 available for public use. .. The cells were derived from human neonatal foreskin fibroblasts (BJ CRL-44 2522, ATCC) with a normal diploid karyotype and reprogrammed using Yamanaka factors via 45 retroviral transduction. .. 46 iPSC culture 47 iPSC cells were maintained in Cellartis DEF-CS 500 Culture System (Y30010, Takara Bio) on DEF-48 CS COAT-1 (Y30012, Takara Bio), diluted 1:20 in PBS with Ca2+ & Mg2+, coated cell culture plates.

    Article Title: Optimized prime editing of the Alzheimer’s disease-associated APOE4 mutation
    Article Snippet: 39 40 Supplemental Experimental Procedures 41 iPSC line derivation 42 The R-iPS 1J cell line (internal identifier) were generated internally at AstraZeneca and not made 43 available for public use. .. The cells were derived from human neonatal foreskin fibroblasts (BJ CRL-44 2522, ATCC) with a normal diploid karyotype and reprogrammed using Yamanaka factors via 45 retroviral transduction. .. 46 iPSC culture 47 iPSC cells were maintained in Cellartis DEF-CS 500 Culture System (Y30010, Takara Bio) on DEF-48 CS COAT-1 (Y30012, Takara Bio), diluted 1:20 in PBS with Ca2+ & Mg2+, coated cell culture plates.

    Transduction:

    Article Title: Optimized prime editing of the Alzheimer’s disease-associated APOE4 mutation
    Article Snippet: iPSC line derivation 42 The R-iPS 1J cell line (internal identifier) were generated internally at AstraZeneca and not made 43 available for public use. .. The cells were derived from human neonatal foreskin fibroblasts (BJ CRL-44 2522, ATCC) with a normal diploid karyotype and reprogrammed using Yamanaka factors via 45 retroviral transduction. .. 46 iPSC culture 47 iPSC cells were maintained in Cellartis DEF-CS 500 Culture System (Y30010, Takara Bio) on DEF-48 CS COAT-1 (Y30012, Takara Bio), diluted 1:20 in PBS with Ca2+ & Mg2+, coated cell culture plates.

    Article Title: Optimized prime editing of the Alzheimer’s disease-associated APOE4 mutation
    Article Snippet: 39 40 Supplemental Experimental Procedures 41 iPSC line derivation 42 The R-iPS 1J cell line (internal identifier) were generated internally at AstraZeneca and not made 43 available for public use. .. The cells were derived from human neonatal foreskin fibroblasts (BJ CRL-44 2522, ATCC) with a normal diploid karyotype and reprogrammed using Yamanaka factors via 45 retroviral transduction. .. 46 iPSC culture 47 iPSC cells were maintained in Cellartis DEF-CS 500 Culture System (Y30010, Takara Bio) on DEF-48 CS COAT-1 (Y30012, Takara Bio), diluted 1:20 in PBS with Ca2+ & Mg2+, coated cell culture plates.



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